<div> <strong>ZnCPT</strong> </div>

Welcome to ZnCPT – The zinc binding cysteine proteome database!

Zinc is an essential micronutrient that regulates a wide range of physiological processes, principally through Zn²⁺ binding to protein cysteine residues. Despite being critical for modulation of protein function, for the vast majority of the human proteome the cysteine sites subject to Zn²⁺ binding remain undefined. Here we develop ZnCPT, a comprehensive and quantitative mapping of the zinc binding cysteine proteome. We define thousands of zinc binding protein cysteines, uncovering protein families across major domains of biology that are subject to either constitutive or inducible modification by zinc. ZnCPT enables systematic discovery of zinc binding structural, enzymatic, and allosteric functional domains. We provide ZnCPT as a resource for understanding mechanisms of zinc regulation of protein function. ZnCPT determines the zinc binding status across HCT116 cells, as well as primary human hepatocytes and prostate epithelial cells.

Please Cite: Burger et al. A comprehensive landscape of the zinc-regulated human proteome. bioRxiv 2024.01.04.574225; doi: https://doi.org/10.1101/2024.01.04.574225

Background

Guide to exploring ZnCPT

Select the cell type in which you would like to explore zinc binding to your favorite protein
Add the UniProt Name, Gene Name or UniProt ID of your favorite protein into the search query box and select the appropriate category under search for
If your protein is not found, likely it was not mapped in the selected dataset. Please ensure you have entered the correct name or ID
The average ratio of labelled cysteines compared to the untreated control (level of accessibility) is provided. Statistical significance between control and treatment is shown.
Under the structure tab quantified cysteines can be explored on a primary sequence and structural level.
Cysteine data for individual proteins can be downloaded from the search window.
The entire dataset can be downloaded from the “Download” window.

Note the cysteine site numbers indicate the cysteine(s) that were labelled for a given peptide.

The ZnCPT web application was developed by Shelley Wei (Chouchani Lab, DFCI) and Nils Burger (Chouchani Lab, DFCI/HMS).

Please Cite: Burger et al. A comprehensive landscape of the zinc-regulated human proteome. bioRxiv 2024.01.04.574225; doi: https://doi.org/10.1101/2024.01.04.574225

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The ZnCPT dataset was done in HCT116 cells, primary human prostate epithelial cells, and primary human hepatocytes. Use the toggle function to choose the cell line you want to search in.

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